Research Centres

LIST OF INSTRUMENTS USED IN THE RESEARCH LABHigh-performance liquid chromatography (sometimes referred to as high-pressure liquid chromatographyis a chromatographic technique that can separate a mixture of compounds and is used in biochemistry and analytical chemistry to identity, quantity and purify the individual components of the mixture. Also used in the study of various plant metabolites.
Atomic absorption spectroscopy is a spectroanalytical procedure for the qualitative and quantitative determination of chemical elements employing the absorption of optical radiation (light) by free atoms in the gaseous state. In analytical chemistry the technique is used for
determining the concentration of a particular element (the analyte) in
a sample to be analyzed. AAS can be used to determine over 70 different
elements in solution or directly in solid samples.

Gas chromatography (GC),is a common type of chromatography used in analytic chemistry for separating and analysing compounds that can be vaporized without decomposition. Typical uses of GC include testing the purity of a particular substance, or separating the different components of a mixture (the relative amounts of such components can also be determined). In some situations, GC may help in identifying a compound.

Polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.

Gel Doc, also known as Gel Documentation System, Gel Image System or Gel Imager, is widely used in molecular biology laboratories for the imaging and documentation of nucleic acid and protein polyacrylamide or agarose gels typically stained with ethidium bromide or other fluorophores. Generally, a Gel Doc is composed of an ultraviolet (UV)light transilluminator, a hood to
shield external light sources and a camera for image capturing.

Ultraviolet-visible spectroscopy or ultraviolet-visible spectrophotometry (UV-Vis or UVIVis) It uses light in the visible and adjacent (near-UV and
near-infrared (NIR)) ranges. The absorption or reflectance in the visible range directly affects the perceived color of the chemicals involved. In this region of the electromagnetic spectrum, molecules undergo electronic transitions. It is used for the analysis of various
biological components.

Equipments Purchased for Various Funded Projects:

Sl No Name of the Equipment
1 Micropipette (0.0 Iml-O.lml range)
2 Homogeniser
3 Microtome
4 Grinding Mill (Junior Grindwell)
5 Rotary evaporator
6 Olympus binocular microscope with photomicrograhic
facility
7 Autoanalyser
6 ELISAreader
8 Deep freezer (-70°C)

Equipment uses:

1. Micropipette (0.01ml-0.1ml range): To pipette,
microliters of samples for biochemical estimations

2. Homogeniser: For the homogenization of tissues.

3. Microtome: Sections of various tissues are taken for the evaluation
of histopathological changes.

4. Grinding Mill: For grinding of plant parts to make it powder for the
extraction.

5. Rotary evaporator: For the evaporation of solvents present in the
extract at low pressure and temperature.

6. Olympus binocular microscope with photomicrograhic facility: For the
microscopic examination of tissue sections and to take photographs of
histopathological changes for documentation.

7. Auto analyser:  Body fluids of experimental animals like
blood/plasma/serum samples are to be subjected for various biochemical
analysis where it is necessary to carryout continuous serum enzyme
assay. As the volume of these samples we obtain from animals may not be
sufficient to get analyzed manually, it is proposed to have an
autoanalyser.

8. ELISA reader: Necessary for biochemical assay of antioxidants for
measuring absorbance continuously.

9. Deep freezer: For pre freezing and storage purpose of the extracts
and animal tissue samples for investigations.

DEPARTMENT OF MECHANICAL ENGINEERING

ONDITION MONITORING LABORATORY

The Condition Monitoring Laboratory setup under VTU Research Grant
Scheme is being used for carrying out research activities related to
vibration monitoring/ vibration signal analysis of antifriction
bearings. The lab consists of a customized bearing test rig, designed
inhouse and fabricated by M/s Balaji Autotech Ltd., Mysore for
conducting experiments on two types of deep groove ball bearings (6205
and 6206) under different loads and speeds (Rs. 1.12Iakhs). Vibration
data acqQisition can be carried out using VIBSCANNERVIB 5.400 – Single
channel vibrometer with FFT analyzer, which is a hand-held device for
vibration signal acquisition, along with the associated
software(OMNITREND) for acquisition and analysis (supplied by M/s
Pruftechnik, Germany, cost Rs. 5.4 lakhs). The other vibration data
acquisition system for continuous on-line measurement is – NI-DAQSystem
consisting of PCI based Sound and Vibration Measurement setup, Signal
Conditioning Module SCXI- 1121 with 4-channel Isolation Amplifier with
Excitation (NI SCXI-1121) and Data Acquisition Bundle with 4 slot
Signal Conditioning chassis (PCI-6221/SCXI-I000 Bundle).

2)   MICRO-ELECTRO-DISCHARGE-MACHING RESEARCH FACILTY

A prototype micro-EDM research facility is developed indigenously.
Pulsed power supply system, workpiece positioning system, tool feed
control system and in situ tool wear and  a~hining depth
measurement systems are developed and integrated in to micro-EDM
system. A 3D micro-EDM process simulation model is to be developed to
estimate the tool wear compensation required to reach the desired depth
of micromachining on workpiece. Nano particles of different sizes can
be generated by controlling gap voltage, pulse-on time, and sparking
frequency. Microchannels can be machined on conducting and
semiconducting materials by providing tool rotations and the effect of
process parameters on machining characteristics can be investigated.


Research facility to study the hydraulic displacement amplification characteristics of a multilayer piezoelectric actuator:

In this research work an effort is made to investigate the displacement
amplification characteristics of a multilayer piezoelectric actuator
using hydraulic amplification principle. An electro-mechanical model is
developed to estimate the displacement of a multilayer piezoelectric
actuator for the applied input voltage considering the hysteresis
behaviour. An embedded controller will be developed for the prototype
actuator based on the developed mathematical model for the prototype
actuator. Hardware-in-loop simulation will be performed for the
different control strategies for the position and tracking control of
the actuator.

DEPARTMENT OF PHYSICS:

Refractive index measurement unit: to measure the Refractive Index of
the single crystals or any transparent materials using Laser

Diode Laser (Green – continuous, 1.0mW, 532nm,): To use as a source to
determine the Refractive Index Laser power meter: To measure the output
power of Laser light  Crystal growth apparatus with constant
temperature bath: To grow quality crystals by solution, slow
evaporation techniques at different temperature conditions Double
distilled water Plant: To get double distilled water used as solvent
for crystal growth Magnetic Stirrer: Used to get homogeneous solution
Polarizing microscope: To study birefringent crystals by obtaining
conoscopic images and to determine the refractive index.